1. Role of the chromatin landscape as molecular determinants of DNA replication origin function
2. Impact of pre-RC protein dynamics on genome replication, cell division and organ growth
3. Role of ploidy and transcriptional programs in genome replication, cell division and organ growth

We have a variety of tools and strategies to tackle these objectives:

• A genome-wide map of ORIs of a whole organism at the post-embryonic stage
• Dynamics of DNA replication initiation proteins
• The last cell cycle before differentiation is unique in its G2 and likely S-phase
• Painting cell cycle phases in toto
• A variety of whole and single-cell genomics